Constrained Multistate Sequence Design for Nucleic Acid Reaction Pathway Engineering
نویسندگان
چکیده
منابع مشابه
Constrained Multistate Sequence Design for Nucleic Acid Reaction Pathway Engineering.
We describe a framework for designing the sequences of multiple nucleic acid strands intended to hybridize in solution via a prescribed reaction pathway. Sequence design is formulated as a multistate optimization problem using a set of target test tubes to represent reactant, intermediate, and product states of the system, as well as to model crosstalk between components. Each target test tube ...
متن کاملSensitivity and Specificity of Nucleic Acid Sequence-Based Amplification Method for Diagnosis of Cutaneous Leishmaniasis
Abstract Background and Objective: Culture, microscopic method is a gold standard method for identification of Lishmania parasite. The use of Molecular methods such as RT- PCR compared to microscopic methods has a higher sensitivity and specificity however, it is not widely used due to its expensive equipment and the time requested. The use of nucleic acid sequence based amplification (NASBA) ...
متن کاملSimulation and Automatic Design of Nucleic Acid Reaction System
I propose an efficient simulation technique for analyzing biological reactions and an automatic design method for developing artificial reactions, both of which are targeted to systems mainly composed of nucleic acids. DNA that is one type of nucleic acids is utilized as a genetic information carrier in biology, while RNA that is the other type of nucleic acids has a biological functionality be...
متن کاملRNAblueprint: flexible multiple target nucleic acid sequence design
Motivation Realizing the value of synthetic biology in biotechnology and medicine requires the design of molecules with specialized functions. Due to its close structure to function relationship, and the availability of good structure prediction methods and energy models, RNA is perfectly suited to be synthetically engineered with predefined properties. However, currently available RNA design t...
متن کاملNucleic acid sequence design via efficient ensemble defect optimization
We describe an algorithm for designing the sequence of one or more interacting nucleic acid strands intended to adopt a target secondary structure at equilibrium. Sequence design is formulated as an optimization problem with the goal of reducing the ensemble defect below a user-specified stop condition. For a candidate sequence and a given target secondary structure, the ensemble defect is the ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of the American Chemical Society
سال: 2017
ISSN: 0002-7863,1520-5126
DOI: 10.1021/jacs.6b12693